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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
doi: 10.1074/jbc.RA118.005676
Figure Lengend Snippet: Production of soluble Siglec-14 is not proportional to the expression level of membrane-bound Siglec-14. A, ELISA of soluble Siglec-14 produced by FLAG-Siglec-14/THP-1 and U937 cell lines. Siglec-14–specific mouse mAb (clone 40-1) was used for detection. B, expression levels of membrane-bound Siglec-14 on FLAG-Siglec-14/THP-1 and U937 cell lines. Solid line, cells stained with anti-Siglec-14 antibody (clone 40-1); gray-shaded area, cells stained with control mouse antibody.
Article Snippet: Cell lines and
Techniques: Expressing, Membrane, Enzyme-linked Immunosorbent Assay, Produced, Staining, Control
Journal: The Journal of Biological Chemistry
Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
doi: 10.1074/jbc.RA118.005676
Figure Lengend Snippet: Human bone marrow cells produce alternatively spliced mRNA encoding soluble Siglec-14. Human bone marrow first-strand cDNA library (A–C) and first-strand cDNA prepared from U937 cells (C) were used as templates. A, agarose gel electrophoresis of 3′RACE PCR products obtained using a gene-specific primer (annealing to exon 5) and a universal primer. Products of nested PCR (second round) were separated by agarose gel electrophoresis. B, agarose gel electrophoresis of RT-PCR products with the primer pair flanking intron 5. C, relative abundances of membrane-bound (solid bars) and soluble (open bars) SIGLEC14 mRNA isoforms, normalized against ACTB mRNA.
Article Snippet: Cell lines and
Techniques: cDNA Library Assay, Agarose Gel Electrophoresis, Nested PCR, Reverse Transcription Polymerase Chain Reaction, Membrane